Physicochemical characterization of the layer-by-layer self-assembly of polyphenol oxidase and chitosan on glassy carbon electrode

LILIANE COCHE-GUERENTE; JACQUES DESBRIERES; JULIEN FATISSON; PIERRE LABBE; MARCELA C. RODRÍGUEZ; GUSTAVO A. RIVAS

ELECTROCHIMICA ACTA

Elsevier

Lugar: Amsterdam; Año: 2005 vol. 50 p. 2865 - 2865

0013-4686

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: justify; mso-layout-grid-align: none">This paper demonstrates the feasibility to build up layer-by-layer (L-b-L) self-assemblies of polyphenol oxidase (PPO) and quaternized chitosan (CHI+) on a glassy carbon (GC) rotating disk electrode. This work highlights the promising properties of this modified polysaccharide to immobilize PPO in self-assembled structures under conditions that preserve its catalytic activity. The film consists of a diffusional underlayer coupled with an outermost active layer of (PPO/CHI+)n. UV–vis spectroscopy and quartz crystal microbalance (QCM) were used to follow the multilayer film formation. Referring to the low amount of enzyme immobilized in the multilayer structure, the amperometric response of the biosensor reached an excellent sensitivity of about 2000Amol